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Research Interest:
Genes, Circuits & Behavior.
Thousands of genes operate in the brain circuits, each at a different time and space, to control complex behavior in an animal. We are using molecular imaging techniques in Drosophila aiming to understand the genetic and neural basis of brain functions. Two main approaches are taken: (i) to reconstruct wiring diagrams of the whole brain; and (ii) to understand computation in specific circuits for innate as well as learned behaviors. We have constructed a standardized Drosophila model brain as a web-based 3D image database for archiving, searching and analysis of individual neurons. This allows a fast and full scale analysis of interconnections between elementary processing units, defined by the presence of population of local neurons, in a complete brain. Such a comprehensive description of inter-regional pathways provides an anatomical road map for the prediction of functional circuits in the Drosophila brain. We focus on understanding the computation process for olfactory, auditory, gustatory, sleep, and memory behaviors. Our goal is to investigate how genes, neurons, and neural circuits contribute to complex behaviors using Drosophila as the model system.
Wiring diagram. We have generated a standard Drosophila brain for archiving and analysis of individual neurons. We are trying to construct (i) a circuitry map showing connections among different neuropilar regions; and (ii) a map of synaptic connectivity among olfactory neurons.
Brain functions. We focus on understanding circuits and genes involving in the computation process for olfactory, auditory, gustatory, sleep, courtship, fighting, aging, fear, and learned behaviors.
Selected Publications :
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Wang Y, Mamiya A, Chiang AS, Zhong Y* (2008) Imaging of an early memory trace in the
Drosophila mushroom body. J Neurosci 28, 4368-76 (IF: 7.453)
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Wu CL, Xia S, Fu TF, Wang H, Chen YH, Leong D, Chiang AS*, Tully T*. Specific requirement of NMDA receptors for long-term memory consolidation in Drosophila ellipsoid body. Nature Neuroscience (Advance Online Publication 10.1038/nn2005) (IF: 15.456) <Link>
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Lin HH, Jason Lai SY, Chin AL, Chen YC, Chiang AS (2007) A Map of Olfactory Representation in the Drosophila Mushroom Body. Cell 128, 1205-1218. (IF: 29.431) <Link>
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Zhu S, Lin S, Kao CF, Awasaki T, Chiang AS, Lee T (2006) Gradients of the Drosophila chinmo BTB-zinc finger protein govern neuronal temporal identity. Cell 127, 409-422. (IF: 29.431) <Link>
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Agrawal N, Pallos J, Slepko N, Apostol BL, Bodai L, Chang LW, Chiang AS, Thompson LM, Marsh JL (2005) Identification of combinatorial drug regimens for treatment of Huntington's disease using Drosophila. Proc. Natl. Acad. Sci. USA 102, 3777-81. (IF: 10.23; Ranking: 6.3%) <Link>
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Xia S, Miyashita T, Fu TF, Lin WY, Wu CL, Pyzocha
L, Lin IR, Saitoe M, Tully T, Chiang AS (2005)
NMDA receptors mediate olfactory learning and memory in Drosophila.
Curr. Biol. 15, 603-615. (IF: 11.91) <Link>
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Agrawal N, Pallos J, Slepko N, Apostol BL, Bodai L, Chang
LW, Chiang AS, Thompson LM, Marsh JL (2005)
Identification of combinatorial drug regimens for treatment
of Huntington’s disease using Drosophila Proc. Natl.
Acad. Sci. USA
102, 3777-81 (IF: 10.272) <Link>
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ijima K, Liu HP, Chiang AS
, Konsolaki M, Zhong Y (2004) A Drosophila model
of Alzheimer's disease: dissecting the pathological roles
of Ab42 and Ab40. Proc. Natl. Acad. Sci . USA 101,
6623-6628. (IF: 10.272) <Link>
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Mignone J, Kukekov V, Chiang AS , Steindler D, Enikolopov G (2004) Neural stem and progenitor cells in nestin-GFP transgenic mice. J. Comp. Neurol. 469, 311-24 (IF: 3.672)
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Tamura T, Chiang AS, Ito
N, Liu HP, Horiuchi J, Tully T, Saitoe M (2003) Aging specifically
impairs amnesiac-dependent memory in Drosophila .
Neuron 40, 1-20. (IF: 15.30) <Link>
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Zhu S, Chiang AS , Lee T (2003)
Development of the Drosophila mushroom bodies: elaboration,
remodeling, and spatial organization of dendrites in the calyx.
Development 130, 2603--2610. (IF: 7.88) <Link>
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Wang Y, Chiang AS, Xia S,
Kitamoto T, Tully T, Zhong Y (2003) Blockade of neurotransmission
in Drosophila mushroom bodies impairs odor attraction,
but not repulsion. Curr . Biol. 13, 1900-1904.
(IF: 11.91) <Link>
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Dubnau J, Chiang AS, Grady
L, Barditch J, Gossweiler S, McNeil J, Smith P, Buldoc F,
Scott R, Certa U, Broger C, Tully T (2003) The staufen
/ pumilio pathway is involved in Drosophila
long-term memory. Curr . Biol. 13, 286-296.
(cover story) (IF: 11.91) <Link>
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Liu YC , Chiang AS * (2003) High-resolution confocal imaging and 3-D rendering. Methods 30, 86-93. (IF: 3.622)
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Dubnau J, Chiang AS , Tully T. (2003) Neural substrates of memory: From synapse to system. J. Neurobiol. 54, 238-253. (IF: 3.145)
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Chiang AS *, Lin WY, Liu HP,
Pszczolkowski MA, Fu TF, Chiu SL, Holbrook GL (2002) Insect
NMDA receptors mediate juvenile hormone biosynthesis. Proc.
Natl. Acad. Sci . USA 99, 37-42. (IF: 10.7) <Link>
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Malaterre J, Strambi C, Chiang AS , Aouane A, Strambi A, Cayre M (2002) Development of cricket mushroom bodies. J. Comp. Neurol. 452, 215-227. (cover story) (IF: 3.672)
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Chiang AS *, Liu YC, Chiu SL, Hu SH, Huang CY, Hsieh CH (2001) Three-dimensional mapping of brain neuropils in the cockroach, Diploptera punctata. J. Comp. Neurol. 440, 1-11. (IF: 3.672)
International invited talks :
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2009/04/14 FlyCircuit: A comprehensive Drosophila brain circuit database. In “Synapses: From
Molecules to Circuits & Behavior”. Cold Spring Harbor Laboratory, NY, USA.
(Session Chair)
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2009/01/11 Three-dimensional reconstruction of wiring diagrams in the Drosophila brain. In “The
6th Asian Biophysical Association (ABA) Symposium”. Hong Kong University of
Science and Technology, Hong Kong (Plenary lecture)
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2008/12/04 Reconstruction of wiring diagrams in the Drosophila brain. Department of Biological
Sciences, National University of Singapore.
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2008/07/10 Building a wiring diagram of the Drosophila brain. Cold Spring Harbor Laboratory,
NY, USA.
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2008/06/30 Building a wiring Diagram of the Drosophila brain. Woods Hole Marine Biological
Laboratory, MA, USA.
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2007/10/18 Taiwanese-Swiss Bilateral Workshop on Nanoscience and Nanotechnology, Zermatt,
Switzerland. (Plenary talk)
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2007/08/14 Mapping olfactory circuits in the Drosophila brain. Department of Biological
Sciences, HKUST, Hong Kong.
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2007/07/10 Building a Wiring Diagram of the Drosophila Brain. Cold Spring Harbor Laboratory, NY, USA
- 2007/06/30 Building a Wiring Diagram of the Drosophila Brain. Woods Hole Marine Biological Laboratory, MA, USA.
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2007/03/12 A Pilot Platform for Archiving 3D Images of the Drosophila Olfactory Circuitry. Janelia Farm--Howard Hughes Medical Institute, Virginia, USA. (Plenary talk)
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2006/07/05
Deconstructing Drosophila Brain Circuits. The Department of Molecular, Cellular and Developmental Biology, Yale University , New Haven , USA
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2006/06/28 Deconstructing Drosophila Brain Circuits. Cold Spring Harbor Laboratory , NY , USA
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2005 Mapping Memory Circuits in the Drosophila Brain. Molecular Basis of Aging and Neuronal Disorder, Ilsong Institute of Life Science, Hallym University, Anyang, Korea
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2005 Mapping Memory Circuits in the Drosophila Brain. Frontiers in Cellular Neuroimaging, RIKEN Brain Science Institute, Wako-shi, Saitama , Japan
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2005 Mapping Memory Circuits in the Drosophila Brain. Seminar in the Tokyo Metropolitan Institute for Neuroscience, Fuchu , Tokyo , Japan
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2005 Mapping Memory Circuits in the Drosophila Brain, Kunmin Institute of Zoology, Chinese Academy of Sciences,
Kunmin, China
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2005 Mapping Memory Circuits in the Drosophila Brain, Institute of Neuroscience, Shanghai Institute for Biological
Sciences, Chinese Academy of Sciences, Shanghai, China
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2004 Mapping Candidate Memory Circuits in
the Brain. Cold Spring Harbor Laboratory, USA.
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2004 High-resolution confocal imaging of
Drosophila brain circuits. 14th International Congress on
Photobiology. Jeju, Korea
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2004 Symposium on Neural Plasticity, Development
and Repair. University of Hong Kong, Hong Kong.
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2003 Fly Brain Neurogenomics: Postdoctoral
Research Possibilities in Taiwan. Department of Bioscience
and Biotechnology, Tsinghua University, Beijing, China.
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2002 Bioinformatics Beyond Sequence: Memory
Circuits and Brain Database. Institute of Neuroscience, Department
of Bioscience and Biotechnology, Tsinghua University, Beijing,
China.
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2002 Visions from CSHL: High-resolution Mapping
of Memory Circuits. Cold Spring Harbor Laboratory, USA.
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2002 High-resolution Confocal Imaging of
Olfactory Memory Circuits in the Drosophila Brain. Department
of Cell & Structural Biology, University of Illinois.
USA.
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2002 High-resolution Confocal Imaging of
Olfactory Memory Circuits in the Drosophila Brain. Department
of Entomology, Department of Entomology Genetics, North Carolina
University. USA
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Aqueous Tissue Clearing Solution. USA Patent No: US 6472216 B1. Taiwan Patent No: 206390. United Kingdom Patent No: GB237757. France Patent No: 2830323
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3D Montage. Taiwan Patent No: 206631. ( USA and China patent pending)
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Bio-Expression System and the Method of the Same ( USA and Taiwan patent pending)
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