Jya-Wei Cheng
程家維教授

Research Interest: 
My group is actively engaged in the determination of biomolecular structures and functions using multi-dimensional NMR spectroscopy and other biophysical techniques (such as CD, fluorescence, Biosensor, etc.).  During the past years, we have made considerable progress in understanding the the stability, folding, structure, and peptide binding specificity of the SH3 and SH2 domains of Btk, a tyrosine kinase that is involved in X-Linked agammaglobulinemia (XLA) and B cell development.  The success of this work depended heavily on the protein cloning, expression, purification, and NMR structural study ability of this group.

In addition to the structural and functional studies of Btk, we have determined the solution conformation of the propeptide domain of factor IX and the predicted a-helix region of HIV-1 integrae.  We have also determined the relationship between local helix contents and RNA binding activity of the N-terminal leucine-repeat region of hepatitis delta antigen.  For the DNA/RNA structural studies, we have determined the hydration and solution structures of the DNA/RNA hybrid duplexes, [d(CGC)r(aaa)d(TTTGCG)]₂ and [d(CGC)r(a_ma_ma_m)d(TTTGCG)]₂.  We have also developed a new synthetic method to synthesize ¹⁵N- and ¹³C-labeled nucleosides which can be used in the protein/DNA complex structural studie.

Selected Publications: 

1. Wu KP, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW, Wu SC, Cheng JW*. Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein Journal of Biological Chemistry 278, 46007-46013 (2003).
   
   
2. Pai MT, Yang CS, Tzeng SR, Wang C, Cheng JW*. 1H, 15N and 13C resonance assignments of the tetratricopeptide repeat (TPR) domain of hSGT Journal of Biomolecular NMR 26, 381-382 (2003).
   
   
3. M.T. Pai, K.C. Huang, S.R. Tzeng, and J.W. Cheng*, 1H, 15N and 13C resonance assignments of the SH2 domain of Bruton's tyrosine kinase Journal of Biomolecular NMR 24, 163-164 (2002).
   
   
4. F.T. Lung, J.Y. Tsai, S.Y. Wei, C. Chen, P.P. Roller, and J.W. Cheng, Novel peptide inhibitors for Grb2 SH2 domain and their detection by surface plasmon resonance Journal of Peptide Research 60, 143-149 (2002).
   
   
5. M.L. Jain, Y.P. Tsao, N.L. Ho, and J.W. Cheng*, A Facile Synthesis of [N1, NH2-15N2]Adenine, [N3, NH2-15N2]Adenine and [N1, N3, NH2-15N2]Adenine Journal of Organic Chemistry 60, 6472-6475 (2001).
   
   
6. Y.P. Tsao, L.Y. Wang, S.T. Hsu, M.L. Jain, S.H. Chou, W.C. Huang, and J.W. Cheng*, The Solution Structure of [d(CGC)r(amamam)d(TTTGCG)]2 Journal of Biomolecular NMR 21, 209-220 (2001).
   
   
7. S.R. Tzeng, M.T. Pai, F.T. Lung, C.W. Wu, P.P. Roller, B. Lei, C.J. Wei, S.C. Tu, S.H. Chen, W.J. Soong, and J.W. Cheng*, Stability and Peptide Binding Specificity of BTK SH2 Domain: Molecular Basis for X-Linked Agammaglobulinemia Protein Science 9, 2377-2385 (2000).
   
   
8. Y.C. Lou, I.J. Lin, M.T. Pai, and J.W. Cheng*, Solution Structure of an N-Capping Peptide from the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen Archives of Biochemistry and Biophysics 377, 219-227 (2000).
   
   
9. S.T. Hsu, M.T. Chou, and J.W. Cheng*, The Solution Structure of [d(CGC)r(aaa)d(TTTGCG)]2: Hybrid Junctions Flanked by DNA Duplexes Nucleic Acids Research 28, 1322-1331 (2000).
   
   
10. S.T. Hsu, M.T. Chou, S.H. Chou, W.C. Huang and J.W. Cheng*, Hydration of [d(CGC)r(aaa)d(TTTGCG)]2 Journal of Molecular Biology 295, 1129-1137 (2000).
    
    
11. S.R. Tzeng, Y.C. Lou, M.T. Pai, and J.W. Cheng*, Solution Structure of the BTK SH3 Domain Complexed with a Proline-Rich Peptide from p120cbl Journal of Biomolecular NMR 16, 303-312 (2000).
    
    
12. Y.C. Lou, F.T. Lung, M.T. Pai, S.R. Tzeng, S.Y. Wei, P.P. Roller, and J.W. Cheng*, Solution Structure and Dynamics of a Nonphosphorelated Cyclic Peptide Inhibitor for the Grb2 SH2 Domain Archives of Biochemistry and Biophysics 372, 309-314 (1999).
    
    
13. S.H. Chou, Y.Y. Tseng, Y.R. Chen, and J.W. Cheng, Structural Studies of Symmetric DNA Undecamers Containing Non-Symmetrical Sheared (PuGAPu):(PyGAPy) Motifs Journal of Biomolecular NMR 14, 157-167 (1999).
    
    
14. I.J. Lin, Y.C. Lou, M.T. Pai, H.N. Wu and J.W. Cheng*, Solution Structure and RNA Binding Activity of the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen PROTEINS: Structure, Function, and Genetics 37, 121-129 (1999).