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Research Interest:
The major goal of this laboratory
is to elucidate the molecular mechanisms of cellular and
molecular response in stressed mammalian cells. Cells in
culture respond to suboptimal conditions by reprogramming
a series of metabolic processes including reorganization
of cytoskeletal systems, alteration of protein phosphorylation,
and induction of stress (heat shock) proteins, etc.
We
are particular interested in knowing the roles of various
cell signaling leading
to the initiation of stress response and/or apoptotic cell
death. We use heat-shock as well as a number of cytotoxic
compounds as stressors and the metabolic responses are monitored
in cells during and recovering from the stress treatment.
Currently, several related research projects are being undertaken
in this laboratory. 1. Stress response in mammalian cells
- Transactivation of Stress Seventies by Protein Phosphatase
Inhibitors in 9L Rat Brain Tumor Cells; 2. Molecular Mechanisms
of Taxol-Induced Apoptosis in Human Leukemic HL-60 cells;
and 3. Molecular Mechanism of Taxol Cytotoxicity in Human
Non-Small Cell Lung Cancer H460 cells.
Selected Publications:
- Shu, C.-W., N.-L.Cheng, W.-M. Chang, T.-L. Tseng
and Y.-K. Lai, Transactivation of hsp70-1/2 in Geldanamycin-Treated
Human Non-Small Cell Lung Cancer H460 Cells: Involvement of Intracellular
Calcium and Protein Kinase C. J. Cell. Biochem. Accepted. (2004)
- Juang J.-L., T.-C. Chen, S.-S. Jiang, C.-A. Hsiung,
W.-C. Chen, G.-W. Chen, S.-M. Lin, J.-H. Lin, S.-C. Chiu and
Y.-K. Lai, Coupling multiplex RT-PCR to a gene chip assay for
sensitive and semiquantitative detection of severe acute respiratory
syndrome-coronavirus.Lab Invest. 84, 1085-1091 (2004)
- Lai, M.-T., K.-L. Huang, W.-M. Chang and Y.-K.
Lai, Geldanamycin Induction of grp78 Requires Activation of Reactive
Oxygen Species via ER Stress Responsive Elements in 9L Rat Brain
Tumor Cells. Cell. Signal. 15, 585-595 (2003)
- Cheng, T.-J., Y.-F. Tseng, W.-M. Chang, M. D.-T.
Chang and Y.-K. Lai, Retaining of the Assembly Capability of
Vimentin Phosphorylated by Mitogen-Activated Protein Kinase-Activated
Protein Kinase-2. J. Cell. Biochem. 79, 589-602 (2003)
- Chang, W.-M., K.-D. Chen, L.-Y. Chen, M.-T. Lai
and Y.-K. Lai, Mitochondrial Calcium-Mediated Reactive Oxygen
Species Are Essential for the Rapid Induction of the grp78 Gene
in 9L Rat Brain Tumour Cells. Cell. Signal. 15, 57-64 (2002)
- Smbaiah, T., K.-Y. King, S.-C. Tsay, N.-W. Mei,
S. Kakimclahi, Y.-K. Lai , C.-H. Lieu and J.-R. Hwu, Synthesis
and immunofluorescence assay of a new biotinylated paclitaxel. Eur. J. Med. Chem. 37, 349-353 (2002)
- Cheng, T.-J., Y.-L. Lin, A.-S. Chiang and Y.-K.
Lai, Association of Protein Phosphatase 2A with its Substrate
Vimentin Intermediate Filaments in 9L Rat Brain Tumor Cells J.
Cell. Biochem. 79, 126-138 (2000)
- Chu, J.-J., C.-D. Chiang, C.S. Rao, W.-M. Chang
and Y.-K. Lai, Establishment and Characterization of a Paclitaxel-Resistant
Human Non-Small Cell Lung Cancer Cell Line. Anticancer Res. 20,
2449-2456 (2000)
- Chen, L.-Y., A.-S. Chiang, J.-J. Hung, H.-I. Hung
and Y.-K. Lai , Thapsigargin-Induced Grp78 Expression is Mediated
by the Increase of Cytosolic Free Calcium in 9L Rat Brain Tumor
Cells. J. Cell. Biochem. 78, 404-416 (2000).(IF: 3.672)
- Chen, K.-D., M.-T. Lai, J.-J. Hung, L.-Y. Chen
and Y.-K. Lai, Activation of p38 Mitogen-Activated Protein Kinase
and Mitochondrial Ca2+-Mediated Oxidative Stress Are Essential
for the Enhanced Expression of grp78 Induced by the Protein Phosphatase
Inhibitors Okadaic Acid and Calyculin A. J. Cell. Biochem. 76,
585-595 (2000)
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