Associate Professor
Ph.D., Texas A&M University, 1993

Cell Culture Bioengineering; Molecular Viral Vaccines

Research Interest: 

Cell Culture Bioengineering. 細胞生物工程Animal cell culture can be used as an in vitro factory system for producing many biologicals such as viral vaccines, therapeutic proteins and monoclonal antibodies, tissue cells and gene therapy vectors. These products usually can not be generated effectively from bacteria or yeasts, since many post-translational modifications, processing, and complicate host cell interactions are required to present their authentic biological functions.

Our interests are currently focused on the cell culture-derived viral vaccines for Japanese encephalitis virus, enterovirus type 71, dengue viruses (based on the cDNA infectious clone) and the gene therapy-based adenovirus, adeno-associated virus, retrovirus and lentivirus vectors. The other area is to study the in vitro microevolution of RNA viruses in cell culture system. We are currently using JEV and Dengue viruses as examples to elucidate the in-vitro evolution and the RNA viruses. Our studies can provide detailed information of investigating the dynamics of heterogeneous virus populations in cell culture systems that are of great concerns to vaccine development and manufacturing.

Molecular Viral Vaccines. 分子病毒疫苗Vaccination as the medical procedure to protect pathogenic agent infection has been a great achievement in human medicines. Example of small pox vaccine has demonstrated a complete eradication of natural pathogen to be possible through an effective vaccination program. Our research is emphasized on applied virus biology studies including viral epitope identification, viral interactions with host immune system, viral virulence, and viral pathogenesis, which are important to develop effective viral vaccines. Current interests are aimed at understanding the neutralizing epitope(s) of flavivirus envelope (E) protein and the interactions with host immune system. The flavivirus E protein is the major target of neutralizing antibodies produced by the host and is sufficient to elicit a protective immune responses. We have successfully identified some of the conformational epitopes with potentiality to induce anti-JEV neutralizing antibodies.

Selected Publications: 

1. Wu SC*, Liu CC, Lian WC (2004) Optimization of microcarrier cell culture for the inactivated enterovirus 71 vaccine development. Vaccine (in press)
   
   
2. Wu SC*, Lin YJ, Chou JW, Lin CW (2004) Construction and characterization of a Fab recombinant protein for Japanese encephalitis virus neutralization. Vaccine (in press)
   
   
3. Wu SC*, Chiang JR, Lin CW (2004) Novel cell adhesive glycosaminoglycan-binding proteins of Japanese encephalitis virus. Biomacromolecules (in press)
   
   
4. Wu SC*, Huang H, Lin CC (2004) Expression and functional characterization of Helicobacter pylori catalase in baculovirus-infected insect cells. Enzyme and Microbial Technology 35 (2004) 482–487
   
   
5. Liu JH, Wu SC, Chen WS, Yen CC, Yang MW, Tsai YC, Chen PM (2004) An Intron Promotes the Anti-bcr-abl Activities of a Retrovirally-Expressed Ribozyme in Chronic Myeloid Leukemia Cells. Biochemical and Biophysical Research Communications 318, 764-772.
   
   
6. Liu CC, Wu SC* (2004) Mosquito and mammalian cells grown on microcarriers for four-serotype dengue virus production: variations in virus titer, plaque morphology, and replication rate. Biotechnology and Bioengineering 85(5):482-8.
   
   
7. Wu SC*, Yu CH, Lin CW, Chu IM (2003) The domain III fragment of Japanese encephalitis virus envelope protein: mouse immunogenicity and liposome adjuvanticity. Vaccine 21, 2516-1522.
   
   
8. Lin CW, Wu SC* (2003) A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interacted with the neutralizing antibody combining sites. Journal of Virology 77, 2600-2606.
   
   
9. Wu KP, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW, Wu SC, Cheng JW. (2003) Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein. Journal of Biological Chemistry 278:46007-13.
   
   
10. Wu SC*, Lin CW, Lee SC, Lian WC (2003) Phenotypic and genotypic characterization of the neurovirulence and neuroinvasiveness of a large-plaque attenuated Japanese encephalitis virus isolate. Microbes and Infection 5, 475-480.
    
    
11. Wu SC*, Lin YJ, Yu CH (2003) Baculovirus-insect cell expression, purification, and immunological studies of the full-length Japanese encephalitis virus envelope protein. Enzyme and Microbial Technology 33, 438-444.
    
    
12. Wu SC*, Chu IM (2003) Editorial: Special issue on the 2002 symposium of Young Asian Biochemical Engineers' Community (YABEC), Taipei, Taiwan. Enzyme and Microbial Technology 33, 331.
    
    
13. Wu, S.-C.*, Huang, G.Y.-L., Liu, J.-H. (2002) Production of retrovirus and adenovirus vectors for gene therapy: a comparative study using microcarrier and stationary cell culture. Biotechnology Progress 18, 617-622.
    
    
14. Wu, S.-C.*, Huang, G.Y.-L. (2002) Stationary and microcarrier cell culture processes for propagating Japanese encephalitis virus. Biotechnology Progress 18, 124-8.
    
    
15. Wu, S.-C.*, C.-W. Lin (2001) Neutralizing peptide ligands selected from phage displayed libraries mimic the conformational epitope on domain III of the Japanese encephalitis virus envelope protein. Virus Research 76, 59-69.
    
    
16. Wu, S.-C.*, S.-C. Lee (2001) Complete nucleotide sequences and cell-line multiplication patterns of the attenuated variant CH2195LA of Japanese encephalitis virus. Virus Research 73, 91-102.
    
    
17. Wu, S.-C.*, G. Y.-L. Huang (2000) Hydrodynamic shear forces increase Japanese encephalitis virus production from microcarrier-grown Vero cells. Bioprocess Engineering 23, 229-233.
    
    
18. Lin, C.-W., S.-C. Wu, S.-C. Lee, K.-S. Cheng (2000) Genetic analysis and clinical evaluation of vaculoating cytotoxin gene A and cytotoxin-associated gene A in Taiwanese Helicobacter pylori isolates from peptic ulcer patients. Scan. Journal of Infectious Diseases 32, 51-57.
    
    
19. Wu, S.-C.* (1999) Inflence of hydrodynamic shear stress on microcarrier-attached cell growth: cell line dependency and surfactant protection. Bioprocess Engineering 21, 201-206.
    
    
20. 吳夙欽 (1999) 動物細胞工業與微載體培養技術的發展 生物產業 10(2), 113-117.
    
    
21. 吳夙欽 (2002) 利用基因工程技術與昆蟲細胞培養來生產日本腦炎病毒套膜蛋白 工程科技通訊 第62期64-44.
    
    
22. 劉家齊 洪偉立 吳夙欽 (2003) 動物細胞產業與生化工程技術的發展 化工技術 第11卷第5期208-220.
    
    
23. 吳夙欽 (2003) 第十章 動物細胞培養 生物產業技術概論 國立清華大學出版社 301-314.